Hands-On Introduction to RNA-seq (Flyer).
This was a “nano-course” offered at OHSU in February 2017 to introduce cell and molecular biology graduate students to RNA-seq data generation and analyses. We combined lectures with hands-on activities. Julja Burchard, Dr. Alex Nechiporuk, and Dr Arie Baratt were co-instructors. I presented on experimental design and analysis considerations. You can see my slides below.
Topic: use of RNAseq for biomedical discovery
- Data generation: RNA QA; library preparation; sequencing
- Data processing: alignment, genome annotation, junctions
- Data analysis: normalization, differential expression, pathway analysis
- Experimental design: sample size, depth of coverage, confounders